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KMID : 0545120070170091558
Journal of Microbiology and Biotechnology
2007 Volume.17 No. 9 p.1558 ~ p.1562
Influence of Pipe Materials and VBNC Cells on Culturable Bacteria in a Chlorinated Drinking Water Model System
Lee Dong-Geun

Park Seong-Joo
Kim Sang-Jong
Abstract
To elucidate the influence of pipe materials on the VBNC (viable but nonculturable) state and bacterial numbers in drinking water, biofilm and effluent from stainless steel, galvanized iron, and polyvinyl chloride pipe wafers were analyzed. Although no HPC (heterotrophic plate count) was detected in the chlorinated influent of the model system, a DVC (direct viable count) still existed in the range between 3- and 4-log cells/ml. Significantly high numbers of HPC and DVC were found both in biofilm and in the effluent of the model system. The pipe material, exposure time, and the season were all relevant to the concentrations of VBNC and HPC bacteria detected. These findings indicate the importance of determining the number of VBNC cells and the type of pipe materials to estimate the HPC concentration in water distribution systems and thus the need of determining a DVC in evaluating disinfection efficiency
KEYWORD
Viable but nonculturable (VBNC), direct viable count (DVC), drinking water, biofilm, pipe material
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